Green Fluorescent Protein Purification Green Fluorescent Protein (GFP) is a relatively new biochemical tool with a wide range of applications in current research. The natural function of GFP is to facilitate the bioluminescence of jellyfish and other coelenterates. Aequorea GFP (from jellyfish) consists of 238 amino acids packed tightly together in a cylindrical structure called a beta-can (characterized by Yang, et. Al.); the fluorophore is situated on the interior of this structure making it inaccessible to solvents. This fluorophore is believed to be stabilized by hydrogen bonding with surrounding amino acid residues; this bonding serves to delocalize charge. In emitting light, the GFP fluorophore undergoes a cycle in which it absorbs a photon, deprotonates to form a phenola....

Number Of Pages: 5

Number Of Words: 1302

Got a term paper due soon and need some help quick? You have come to the right place. Essay Galaxy has over 50,000 essays, papers, and reports available for download right now! Get ideas from thousands students just like you to help make that essay perfect. Don't hesitate. Join today and get instant access to our enormous database.

Membership Options Price Savings   1 month subscription    $19.95 recurring * 3 months subscription    $39.95 recurring $19.90 6 months subscription    $69.95 non-recurring $49.75