Permeability of Hydrophilic Investigations of the integrity and transport characteristics of 2/4/A1 cells have been done in this report. The cell line was isolated from rat fetal intestinal epithelial cells and transfected with thermolabile SV40 large T antigen. These cells proliferated at 33 °C, but eliminated the antigen and ceased proliferating at a non-permissive temperature (39°C). At 39°C 2/4/A1 cells started to differentiate but simultaneously the cells also underwent massive cell death. When cultured at 37°C these cells formed confluent and tight monolayers that seemed to have paracellular transport characteristics similar to that of the human intestine. Transmission electron microscopy confirmed the development of multilayers at 33°C, monolayers at 37°C and defects in the cell layer due to apoptosis at 39°C. Different immunost....

Number Of Pages: 13

Number Of Words: 3402

Got a term paper due soon and need some help quick? You have come to the right place. Essay Galaxy has over 50,000 essays, papers, and reports available for download right now! Get ideas from thousands students just like you to help make that essay perfect. Don't hesitate. Join today and get instant access to our enormous database.

Membership Options Price Savings   1 month subscription    $19.95 recurring * 3 months subscription    $39.95 recurring $19.90 6 months subscription    $69.95 non-recurring $49.75