ABSTRACT Proteins are the macromolecules that are responsible for most of the bodily functions. By investigating an individual protein, one can be able to understand the functions and structure of an organism. Before this can be done, protein has to be separated from cell components. Using the methods of centrifugation and gel electrophoresis, not only a protein can be separated from cellular components but also from other proteins. An experiment was designed to separate protein contained in liver tissue of a cow based on it's molecular weight. From the centrifugation, soluble protein concentration extracted for the electrophoresis.