Clostridium Difficile and Psuedomembranous Colitis Clostridium difficle is a rod shaped gram positive bacteria. Clostridium difficile is anaerobic and also a spore former, which allows it to live in the environment for months until it is orally transmitted into its host. Clostridium difficile causes psuedomembranous colitis, and antibiotic associated colitis. It produces three toxins Cytotoxin or toxin B which causes cell death, Enterotoxin or toxin A that causes cellular damage and inflammation of the colon, and a third toxin causing increased myoelectric activity in the colon, which causes diarrhea.